Simple and accurate transcriptional start site identification using Smar2C2 and examination of conserved promoter features.

ABSTRACT: The precise and accurate identification and quantification of transcriptional start sites (TSSs) is key to understanding the control of transcription. The core promoter consists of the TSS and proximal non-coding sequences, which are critical in transcriptional regulation. Therefore, the accurate identification of TSSs is important for understanding the molecular regulation of transcription. Existing protocols for TSS identification are challenging and expensive, leaving high-quality data available for a small subset of organisms. This sparsity of data impairs study of TSS usage across tissues or in an evolutionary context. To address these shortcomings, we developed Smart-Seq2 Rolling Circle to Concatemeric Consensus (Smar2C2), which identifies and quantifies TSSs and transcription termin

SUBMITTER: Murray A 

PROVIDER: S-EPMC9827901 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature