Exosomal proteomics identifies RAB13 as a potential regulator of metastasis for HCC.

ABSTRACT:

Background

Exosomal proteins from cancer cells are becoming new biomarkers for cancer monitoring and efficacy evaluation. However, their biological function and molecular mechanism underlying tumor metastasis are largely unknown.

Methods

Bioinformatic methods such as bulk gene expression analysis, single-cell RNA sequencing data analysis, and gene set enrichment analysis were employed to identify metastasis-associated proteins. The in vitro and in vivo experiments were used to investigate the function of RAB13 in HCC metastasis.

Results

We identified RAB13 as one of the critical regulators of metastasis in HCC-derived exosomes for the first time. In vitro, the invasiveness of HCC cell lines could be attenuated by RAB13 silence. In vivo, tumor size and proportion of hi

SUBMITTER: Huang XY 

PROVIDER: S-EPMC9827969 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature