Loss of tetraspanin-7 expression reduces pancreatic β-cell exocytosis Ca²⁺ sensitivity but has limited effect on systemic metabolism.

ABSTRACT:

Background

Tetraspanin-7 (Tspan7) is an islet autoantigen involved in autoimmune type 1 diabetes and known to regulate β-cell L-type Ca²⁺ channel activity. However, the role of Tspan7 in pancreatic β-cell function is not yet fully understood.

Methods

Histological analyses were conducted using immunostaining. Whole-body metabolism was tested using glucose tolerance test. Islet hormone secretion was quantified using static batch incubation or dynamic perifusion. β-cell transmembrane currents, electrical activity and exocytosis were measured using whole-cell patch-clamping and capacitance measurements. Gene expression was studied using mRNA-sequencing and quantitative PCR.

Results

Tspan7 is expressed in insulin-containing granules of pancreatic β-cells and glu

SUBMITTER: McLaughlin K 

PROVIDER: S-EPMC9828109 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature