Beta cell endoplasmic reticulum stress drives diabetes in the KINGS mouse without causing mass beta cell loss.

ABSTRACT:

Aims

Beta cell endoplasmic reticulum (ER) stress can cause cellular death and dysfunction and has been implicated in the pathogenesis of diabetes. Animal models of beta cell ER stress are critical in further understanding this and for testing novel diabetes therapeutics. The KINGS mouse is a model of beta cell ER stress driven by a heterozygous mutation in Ins2. In this study, we investigated how beta cell ER stress in the KINGS mouse drives diabetes.

Methods

We investigated whether the unfolded protein response (UPR) was activated in islets isolated from male and female KINGS mice and whether this impacted beta cell mass and turnover.

Results

Whilst the UPR was up-regulated in KINGS islets, with increased protein expression of markers of all three UPR arms, this was

SUBMITTER: Daniels Gatward LF 

PROVIDER: S-EPMC9828143 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature