In vitro measurement of concentration of unlabeled protein within a hyaluronic acid matrix.

ABSTRACT: There are currently more than 560 therapeutic monoclonal antibodies (mAbs) at various stages of research and clinical testing, including candidates for administration by subcutaneous (SC) injection. Preclinical studies based on in vitro measurements of high molecular weight proteins within simulated SC matrices are assisting laboratory studies of interactions of injectable biotherapeutic proteins within the SC environment in relation to bioavailability. We report a new method for directly measuring diffusion of unlabeled, high molecular weight proteins injected into an in vitro matrix that simulates the negatively charged environment of the SC. The matrix consists of 10 mg/ml HA in a repurposed cell culture chamber. The measurement consists of pipetting triplicate 20 μl protein samples int

SUBMITTER: Dos Santos ACF 

PROVIDER: S-EPMC9828174 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature