The carboxy-terminal tail of GLR3.3 is essential for wound-response electrical signaling.

ABSTRACT: Arabidopsis Clade 3 GLUTAMATE RECEPTOR-LIKEs (GLRs) are primary players in wound-induced systemic signaling. Previous studies focused on dissecting their ligand-activated channel properties involving extracellular and membrane-related domains. Here, we report that the carboxy-terminal tails (C-tails) of GLRs contain key elements controlling their function in wound signaling. GLR3.3 without its C-tail failed to rescue the glr3.3a mutant. We carried out a yeast two-hybrid screen to identify the C-tail interactors. We performed functional studies of the interactor by measuring electrical signals and defense responses. Then we mapped their binding sites and evaluated the impact of the sites on GLR functions. IMPAIRED SUCROSE INDUCTION 1 (ISI1) interacted with GLR3.3. Enhanced electrical activi

SUBMITTER: Wu Q 

PROVIDER: S-EPMC9828246 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature