RHD genotyping to resolve weak and discrepant RhD patient phenotypes.

ABSTRACT:

Background

We instituted RHD genotyping in our transfusion service for obstetrical patients and transfusion candidates. We sought to examine how RHD genotyping resolved weak or discrepant automated microplate direct agglutination (MDA) RhD phenotypings and impacted needs for Rh Immune Globulin (RhIG) and D-negative RBCs.

Study design and methods

We investigated RhD phenotypes with equivocal or reagent-discrepant automated MDA (Immucor, Norcross, GA), weak-2+ immediate-spin tube typings, historically discrepant RhD typings, or D+ typings with anti-D. We performed microarray RHD genotyping (RHD BeadChip, Immucor BioArray Solutions, Warren, NJ). Patients were managed as D+ with weak-D types 1, 2, and 3, and as D-negative with all other results.

Results

Our weak-D prevale

SUBMITTER: Barriteau CM 

PROVIDER: S-EPMC9828470 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature