Substantial gradient mitigation in simulated large-scale bioreactors by optimally placed multiple feed points.

ABSTRACT: The performance of large-scale stirred tank and bubble column bioreactors is often hindered by insufficient macromixing of feeds, leading to heterogeneities in pH, substrate, and oxygen, which complicates process scale-up. Appropriate feed placement or the use of multiple feed points could improve mixing. Here, theoretically optimal placement of feed points was derived using one-dimensional diffusion equations. The utility of optimal multipoint feeds was evaluated with mixing, pH control, and bioreaction simulations using three-dimensional compartment models of four industrially relevant bioreactors with working volumes ranging from 8 to 237 m³ . Dividing the vessel axially in equal-sized compartments and locating a feed point or multiple feed points symmetrically in each compar

SUBMITTER: Losoi P 

PROVIDER: S-EPMC9828524 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature