Pilot scale production and characterization of next generation high molecular weight and tense quaternary state polymerized human hemoglobin.

ABSTRACT: Polymerized human hemoglobin (PolyhHb) is being studied as a possible red blood cell (RBC) substitute for use in scenarios where blood is not available. While the oxygen (O₂ ) carrying capacity of PolyhHb makes it appealing as an O₂ therapeutic, the commercial PolyhHb PolyHeme® (Northfield Laboratories Inc.) was never approved for clinical use due to the presence of large quantities of low molecular weight (LMW) polymeric hemoglobin (Hb) species (<500 kDa), which have been shown to elicit vasoconstriction, systemic hypertension, and oxidative tissue injury in vivo. Previous bench-top scale studies in our lab demonstrated the ability to synthesize and purify PolyhHb using a two-stage tangential flow filtration purification process to remove almost all undesirable Hb sp

SUBMITTER: Cuddington CT 

PROVIDER: S-EPMC9828582 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature