Rieske FeS overexpression in tobacco provides increased abundance and activity of cytochrome b₆ f.

ABSTRACT: Photosynthesis is fundamental for plant growth and yield. The cytochrome b₆ f complex catalyses a rate-limiting step in thylakoid electron transport and therefore represents an important point of regulation of photosynthesis. Here we show that overexpression of a single core subunit of cytochrome b₆ f, the Rieske FeS protein, led to up to a 40% increase in the abundance of the complex in Nicotiana tabacum (tobacco) and was accompanied by an enhanced in vitro cytochrome f activity, indicating a full functionality of the complex. Analysis of transgenic plants overexpressing Rieske FeS by the light-induced fluorescence transients technique revealed a more oxidised primary quinone acceptor of photosystem II (Q_A ) and plastoquinone pool and faster electron trans

SUBMITTER: Heyno E 

PROVIDER: S-EPMC9828649 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature