Elevated CO₂ induces rapid dephosphorylation of plasma membrane H⁺ -ATPase in guard cells.

ABSTRACT: Light induces stomatal opening, which is driven by plasma membrane (PM) H⁺ -ATPase in guard cells. The activation of guard-cell PM H⁺ -ATPase is mediated by phosphorylation of the penultimate C-terminal residue, threonine. The phosphorylation is induced by photosynthesis as well as blue light photoreceptor phototropin. Here, we investigated the effects of cessation of photosynthesis on the phosphorylation level of guard-cell PM H⁺ -ATPase in Arabidopsis thaliana. Immunodetection of guard-cell PM H⁺ -ATPase, time-resolved leaf gas-exchange analyses and stomatal aperture measurements were carried out. We found that light-dark transition of leaves induced dephosphorylation of the penultimate residue at 1 min post-transition. Gas-exchange analyses co

SUBMITTER: Ando E 

PROVIDER: S-EPMC9828774 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature