Molecular homing and retention of muscle membrane stabilizing copolymers by non-invasive optical imaging in vivo.

ABSTRACT: First-in-class membrane stabilizer Poloxamer 188 (P188) has been shown to confer membrane protection in an extensive range of clinical conditions; however, elements of the systemic distribution and localization of P188 at the organ, tissue, and muscle fiber levels in vivo have not yet been elucidated. Here we used non-invasive fluorescence imaging to directly visualize and track the distribution and localization of P188 in vivo. The results demonstrated that the Alx647 probe did not alter the fundamental properties of P188 to protect biological membranes. Distribution kinetics in mdx mice demonstrated that Alx647 did not interface with muscle membranes and had fast clearance kinetics. In contrast, the distribution kinetics for P188-Alx647 was significantly slower, indicating

SUBMITTER: Batti Angulski AB 

PROVIDER: S-EPMC9829555 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature