Microsecond cell triple-sorting enabled by multiple pulse irradiation of femtosecond laser.

ABSTRACT: Femtosecond-laser-assisted cell manipulation, as one of the high throughput cell sorting techniques, is tailored for single-step multiple sorting based on controllable impulsive force. In this paper, femtosecond laser pulses are focused within a pocket structure and they induce an impulse force acting on the flowing objects. The impulsive force is shown to be controllable by a new method to adjust the femtosecond pulse properties. This allows precise streamline manipulation of objects having various physical qualities (e.g., weight and volume). The pulse energy, pulse number, and pulse interval of the femtosecond laser are altered to determine the impulsive force strength. The method is validated in single cell or bead triple-sorting experiments and its capability to perform streamline man

SUBMITTER: Kiya R 

PROVIDER: S-EPMC9829734 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature