Circ_0000799 promotes proliferation and invasion in colorectal cancer and epithelial-mesenchymal transition.

ABSTRACT:

Background

The current study aimed to investigate the effect of circ_0000799 on the biological function of colorectal cancer (CRC) cells and its mechanism.

Methods

First, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was employed for detecting the expression of circ_0000799, miR-647, and miR-1243 in surgically resected specimens from hospitalized CRC patients, CRC-adjacent normal tissues (Normal group), human normal colon epithelial cells (FHC group), and CRC cell lines (HCT116, HT29, SW480, SW620). The cell proliferation, viability, and invasion were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation assay, transwell assay in HCT116 and SW480 cells with overexpression or inhib

SUBMITTER: Geng CH 

PROVIDER: S-EPMC9830346 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature