MicroRNA-543 controls pancreatic cancer development by LINC00847-microRNA-543-STK31 axis.

ABSTRACT:

Background

Pancreatic cancer (PC) is one of the most malignant cancers of the gastrointestinal tract. However, the study of targeted therapy research in PC is not very thorough. Therefore, targeted molecular markers are needed to aid in the diagnosis and treatment of PC.

Methods

In our research, we investigated the biological functions and molecular mechanism of microRNA-543 in PC. Western blotting (WB) and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to analyze the transcription and protein expression of microRNA-543, Serine/threonine kinase 31 (STK31), and LINC00847 in BxPC-3 and PANC-1 cells. Subsequently, Cell Counting Kit-8 (CCK-8), Transwell, colony formation, and flow cytometry (FCM) assays were utilized to evaluate cell growth, migration,

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9830362 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature