A computational approach to identify efficient RNA cleaving 10-23 DNAzymes.

ABSTRACT: DNAzymes are short pieces of DNA with catalytic activity, capable of cleaving RNA. DNAzymes have multiple applications as biosensors and in therapeutics. The high specificity and low toxicity of these molecules make them particularly suitable as therapeutics, and clinical trials have shown that they are effective in patients. However, the development of DNAzymes has been limited due to the lack of specific tools to identify efficient molecules, and users often resort to time-consuming/costly large-scale screens. Here, we propose a computational methodology to identify 10-23 DNAzymes that can be used to triage thousands of potential molecules, specific to a target RNA, to identify those that are predicted to be efficient. The method is based on a logistic regression and can be trained to in

SUBMITTER: Pine AC 

PROVIDER: S-EPMC9830538 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature