Long-term cell fate and functional maintenance of human hepatocyte through stepwise culture configuration.

ABSTRACT: Human hepatocyte culture system represents by far the most physiologically relevant model for our understanding of liver biology and diseases; however, its versatility has been limited due to the rapid and progressive loss of genuine characteristics, indicating the inadequacy of in vitro milieu for fate maintenance. This study, therefore, is designed to define environmental requirements necessary to sustain the homeostasis of terminally differentiated hepatocytes. Our study reveals that the supplementation of dimethyl sulfoxide (DMSO) is indispensable in mitigating fate deterioration and promoting adaptation to the in vitro environment, resulting in the restoration of tight cell-cell contact, cellular architecture, and polarity. The morphological recovery was overall accompanied by the res

SUBMITTER: Sugahara G 

PROVIDER: S-EPMC9830592 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature