Single-cell transcriptomics reveals a mechanosensitive injury signaling pathway in early diabetic nephropathy.

ABSTRACT:

Background

Diabetic nephropathy (DN) is the leading cause of end-stage renal disease, and histopathologic glomerular lesions are among the earliest structural alterations of DN. However, the signaling pathways that initiate these glomerular alterations are incompletely understood.

Methods

To delineate the cellular and molecular basis for DN initiation, we performed single-cell and bulk RNA sequencing of renal cells from type 2 diabetes mice (BTBR ob/ob) at the early stage of DN.

Results

Analysis of differentially expressed genes revealed glucose-independent responses in glomerular cell types. The gene regulatory network upstream of glomerular cell programs suggested the activation of mechanosensitive transcriptional pathway MRTF-SRF predominantly taking place in mesan

SUBMITTER: Liu S 

PROVIDER: S-EPMC9830686 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature