Multi-omics profiles of the intestinal microbiome in irritable bowel syndrome and its bowel habit subtypes.

ABSTRACT:

Background

Irritable bowel syndrome (IBS) is a common gastrointestinal disorder that is thought to involve alterations in the gut microbiome, but robust microbial signatures have been challenging to identify. As prior studies have primarily focused on composition, we hypothesized that multi-omics assessment of microbial function incorporating both metatranscriptomics and metabolomics would further delineate microbial profiles of IBS and its subtypes.

Methods

Fecal samples were collected from a racially/ethnically diverse cohort of 495 subjects, including 318 IBS patients and 177 healthy controls, for analysis by 16S rRNA gene sequencing (n = 486), metatranscriptomics (n = 327), and untargeted metabolomics (n = 368). Differentially abundant microbes, predicted genes, transcri

SUBMITTER: Jacobs JP 

PROVIDER: S-EPMC9830758 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature