Comparative proteomic analysis of seminal plasma exosomes in buffalo with high and low sperm motility.

ABSTRACT:

Background

Exosomes are nanosized membranous vesicles secreted by various types of cells, which facilitate intercellular communication by transporting bioactive compounds. Exosomes are abundant in biological fluids including semen, and their protein composition and the potential of seminal plasma exosomes (SPEs) as fertility biomarkers were elucidated in humans, however, little information is available regarding buffalo (Bubalus bubalis). Here, we examined protein correlation between spermatozoa, seminal plasma (SP), and SPEs, and we compared and analyzed protein differences between high-motility (H-motility) and low-motility (L-motility) SPEs in buffalo.

Results

SPEs were concentrated and purified by ultracentrifugation combined with sucrose density gradient centrifugation,

SUBMITTER: Yu K 

PROVIDER: S-EPMC9830767 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature