Apicobasal transferrin receptor localization and trafficking in brain capillary endothelial cells.

ABSTRACT: The detailed mechanisms by which the transferrin receptor (TfR) and associated ligands traffic across brain capillary endothelial cells (BECs) of the CNS-protective blood-brain barrier constitute an important knowledge gap within maintenance and regulation of brain iron homeostasis. This knowledge gap also presents a major obstacle in research aiming to develop strategies for efficient receptor-mediated drug delivery to the brain. While TfR-mediated trafficking from blood to brain have been widely studied, investigation of TfR-mediated trafficking from brain to blood has been limited. In this study we investigated TfR distribution on the apical and basal plasma membranes of BECs using expansion microscopy, enabling sufficient resolution to separate the cellular plasma membranes of these mo

SUBMITTER: Nielsen SSE 

PROVIDER: S-EPMC9830855 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature