Knockout of secretin ameliorates biliary and liver phenotypes during alcohol-induced hepatotoxicity.

ABSTRACT:

Background

Alcohol-related liver disease (ALD) is characterized by ductular reaction (DR), liver inflammation, steatosis, fibrosis, and cirrhosis. The secretin (Sct)/secretin receptor (SR) axis (expressed only by cholangiocytes) regulates liver phenotypes in cholestasis. We evaluated the role of Sct signaling on ALD phenotypes.

Methods

We used male wild-type and Sct^-/- mice fed a control diet (CD) or ethanol (EtOH) for 8 wk. Changes in liver phenotypes were measured in mice, female/male healthy controls, and patients with alcoholic cirrhosis. Since Cyp4a10 and Cyp4a11/22 regulate EtOH liver metabolism, we measured their expression in mouse/human liver. We evaluated: (i) the immunoreactivity of the lipogenesis enzyme elongation of very-long-chain fatty acids 1 (Elo

SUBMITTER: Kyritsi K 

PROVIDER: S-EPMC9830859 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature