Host protein kinases required for SARS-CoV-2 nucleocapsid phosphorylation and viral replication.

ABSTRACT: Multiple coronaviruses have emerged independently in the past 20 years that cause lethal human diseases. Although vaccine development targeting these viruses has been accelerated substantially, there remain patients requiring treatment who cannot be vaccinated or who experience breakthrough infections. Understanding the common host factors necessary for the life cycles of coronaviruses may reveal conserved therapeutic targets. Here, we used the known substrate specificities of mammalian protein kinases to deconvolute the sequence of phosphorylation events mediated by three host protein kinase families (SRPK, GSK-3, and CK1) that coordinately phosphorylate a cluster of serine and threonine residues in the viral N protein, which is required for viral replication. We also showed that loss or

SUBMITTER: Yaron TM 

PROVIDER: S-EPMC9830954 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature