Rectal swabs as a viable alternative to faecal sampling for the analysis of gut microbiota functionality and composition.

ABSTRACT: Faecal or biopsy samples are frequently used to analyse the gut microbiota, but issues remain with the provision and collection of such samples. Rectal swabs are widely-utilised in clinical practice and previous data demonstrate their potential role in microbiota analyses; however, studies to date have been heterogenous, and there are a particular lack of data concerning the utility of swabs for the analysis of the microbiota's functionality and metabolome. We compared paired stool and rectal swab samples from healthy individuals to investigate whether rectal swabs are a reliable proxy for faecal sampling. There were no significant differences in key alpha and beta diversity measures between swab and faecal samples, and inter-subject variability was preserved. Additionally, no significant

SUBMITTER: Radhakrishnan ST 

PROVIDER: S-EPMC9831010 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature