Comparative gene expression analysis of stemness between periodontal ligament and umbilical cord tissues in humans.

ABSTRACT:

Background/purpose

Due to their regenerative potential, periodontal ligament (PDL) and umbilical cord (UBC) tissues are an attractive potential mesenchymal stem cells (MSCs) source. This study compared the expression patterns of genes related to stemness between fresh PDL and UBC tissues.

Materials and methods

PDL tissues were collected from 38 permanent premolars extracted for orthodontic purposes, and UBC tissues were obtained from three newborns. Each sample was immediately frozen to prevent RNA degradation. cDNA microarray analysis, quantitative real-time polymerase chain reaction (PCR), and immunohistochemical staining were performed. Gene expression patterns associated with dental stemness (DS) and induced pluripotent stemness (iPS) were compared between PDL and UBC ti

SUBMITTER: Lee HJ 

PROVIDER: S-EPMC9831792 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature