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Biodegradation of cefalexin by two bacteria strains from sewage sludge.

ABSTRACT: Bioremediation has been used as an environmentally-friendly, energy-saving and efficient method for removing pollutants. However, there have been very few studies focusing on the specific antibiotic-degrading microorganisms in the activated sludge and their degradation mechanism. Two strains of cefalexin-degrading bacteria (Rhizobium sp. (CLX-2) and Klebsiella sp. (CLX-3)) were isolated from the activated sludge in this study. They were capable of rapidly eliminating over 99% of cefalexin at an initial concentration of 10 mg l^-1 within 12 h. The exponential phase of cefalexin degradation happened a little earlier than that of bacterial growth. The first-order kinetic model could elucidate the biodegradation process of cefalexin. The optimized environmental temperature and pH values for rapid biodegradation by these two strains were found to be 30°C and 6.5-7, respectively. Furthermore, two major biodegradation metabolites of CLX-3, 7-amino-3-cephem-4-carboxylic acid and 2-hydroxy-3-phenyl pyrazine were identified using UHPLC-MS and the biodegradation pathway of cefalexin was proposed. Overall, the results showed that Rhizobium sp. (CLX-2) and Klebsiella sp. (CLX-3) could possibly be useful resources for antibiotic pollution remediation.

SUBMITTER: Tian J

PROVIDER: S-EPMC9832293 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

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Biodegradation of cefalexin by two bacteria strains from sewage sludge.

Tian Jichen J Chen Chong C Lartey-Young George G Ma Limin L

Royal Society open science 20230111 1

Bioremediation has been used as an environmentally-friendly, energy-saving and efficient method for removing pollutants. However, there have been very few studies focusing on the specific antibiotic-degrading microorganisms in the activated sludge and their degradation mechanism. Two strains of cefalexin-degrading bacteria (<i>Rhizobium</i> sp. (CLX-2) and <i>Klebsiella</i> sp. (CLX-3)) were isolated from the activated sludge in this study. They were capable of rapidly eliminating over 99% of ce ...[more]

PMID: 36686552

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Project description:BackgroundMassive amounts of sewage sludge are generated during biological sewage treatment and are commonly subjected to anaerobic digestion, land application, and landfill disposal. Concurrently, persistent organic pollutants (POPs) are frequently found in sludge treatment and disposal systems, posing significant risks to both human health and wildlife. Metabolically versatile microorganisms originating from sewage sludge are inevitably introduced to sludge treatment and disposal systems, potentially affecting the fate of POPs. However, there is currently a dearth of comprehensive assessments regarding the capability of sewage sludge microbiota from geographically disparate regions to attenuate POPs and the underpinning microbiomes.ResultsHere we report the global prevalence of organohalide-respiring bacteria (OHRB) known for their capacity to attenuate POPs in sewage sludge, with an occurrence frequency of ~50% in the investigated samples (605 of 1186). Subsequent laboratory tests revealed microbial reductive dechlorination of polychlorinated biphenyls (PCBs), one of the most notorious categories of POPs, in 80 out of 84 sludge microcosms via various pathways. Most chlorines were removed from the para- and meta-positions of PCBs; nevertheless, ortho-dechlorination of PCBs also occurred widely, although to lower extents. Abundances of several well-characterized OHRB genera (Dehalococcoides, Dehalogenimonas, and Dehalobacter) and uncultivated Dehalococcoidia lineages increased during incubation and were positively correlated with PCB dechlorination, suggesting their involvement in dechlorinating PCBs. The previously identified PCB reductive dehalogenase (RDase) genes pcbA4 and pcbA5 tended to coexist in most sludge microcosms, but the low ratios of these RDase genes to OHRB abundance also indicated the existence of currently undescribed RDases in sewage sludge. Microbial community analyses revealed a positive correlation between biodiversity and PCB dechlorination activity although there was an apparent threshold of community co-occurrence network complexity beyond which dechlorination activity decreased.ConclusionsOur findings that sludge microbiota exhibited nearly ubiquitous dechlorination of PCBs indicate widespread and nonnegligible impacts of sludge microbiota on the fate of POPs in sludge treatment and disposal systems. The existence of diverse OHRB also suggests sewage sludge as an alternative source to obtain POP-attenuating consortia and calls for further exploration of OHRB populations in sewage sludge. Video Abstract.

Dataset Information

Biodegradation of cefalexin by two bacteria strains from sewage sludge.

Publications

Biodegradation of cefalexin by two bacteria strains from sewage sludge.

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