Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene editing analysis in cotton.

ABSTRACT: CRIPSR/Cas9 gene editing system is an effective tool for genome modification in plants. Multiple target sites are usually designed and the effective target sites are selected for editing. Upland cotton (Gossypium hirsutum L., hereafter cotton) is allotetraploid and is commonly considered as difficult and inefficient to transform, it is important to select the effective target sites that could result in the ideal transgenic plants with the CRISPR-induced mutations. In this study, Agrobacterium rhizogenes-mediated hairy root method was optimized to detect the feasibility of the target sites designed in cotton phytoene desaturase (GhPDS) gene. A. rhizogenes showed the highest hairy root induction (30%) when the bacteria were cultured until OD₆₀₀ reached

SUBMITTER: Zhou L 

PROVIDER: S-EPMC9832336 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature