Sequential Cross-linking of Gallic Acid-Functionalized GelMA-Based Bioinks with Enhanced Printability for Extrusion-Based 3D Bioprinting.

ABSTRACT: The printability of a photocross-linkable methacrylated gelatin (GelMA) bioink with an extrusion-based 3D bioprinter is highly affected by the polymer concentration and printing temperature. In this work, we developed a gallic acid (GA)-functionalized GelMA ink to improve the printability at room and physiological temperatures and to enable tissue adhesion and antioxidant properties. We introduced a sequential cross-linking approach using catechol-Fe³⁺ chelation, followed by photocross-linking. The results show that the ink formulation with 0.5% (w/v) Fe³⁺ in GelMA (30% modification) with 10% GA (GelMA30GA-5Fe) provided the optimum printability, shape fidelity, and structural integrity. The dual network inside the printed constructs significantly enhanced the viscoela

SUBMITTER: Jongprasitkul H 

PROVIDER: S-EPMC9832479 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature