Safety Profile of Lutein- Versus Triamcinolone Acetonide-Based Vitreous Staining.

ABSTRACT:

Purpose

To assess the safety profile of a new lutein-based vitreous dye (LB-VD) formulation compared with various triamcinolone acetonide (TA) formulations with and without subsequent exposure to perfluorodecalin (PFD) in vitro.

Methods

Human adult retinal pigment epithelial cells (ARPE-19) were treated with the following formulations: undiluted preserved TA (TA-BA), diluted preserved TA (D-TA-BA), preservative-free TA (TA-PF), and LB-VD. First, cell tolerability was evaluated with MTT, LDH, and ATPlite assays after 1, 5, and 30 minutes of exposure to each tested formulation. Then, cells were sequentially exposed to formulations and PFD. After 24 hours of exposure to PFD, cell tolerability was evaluated through MTT and ATPlite assays.

Results

Among the formulations te

SUBMITTER: Lazzara F 

PROVIDER: S-EPMC9832719 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature