Assessment of Murine Retinal Acuity Ex Vivo Using Multielectrode Array Recordings.

ABSTRACT:

Purpose

Visual acuity, measured by resolution of optotypes on a standard eye chart, is a critical clinical test for function of the visual system in humans. Behavioral tests in animals can be used to estimate visual acuity. However, such tests may be limited in the study of mutants or after synthetic vision restoration techniques. Because the total response of the retina to a visual scene is encoded in spiking patterns of retinal ganglion cells, it should be possible to estimate visual acuity in vitro from the retina by analyzing retinal ganglion cell output in response to test stimuli.

Methods

We created a method, EyeCandy, that combines a visual stimulus-generating engine with analysis of multielectrode array retinal recordings via a machine learning approach to measure mu

SUBMITTER: Babino D 

PROVIDER: S-EPMC9832724 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature