AssemblyTron: flexible automation of DNA assembly with Opentrons OT-2 lab robots.

ABSTRACT: As one of the newest fields of engineering, synthetic biology relies upon a trial-and-error Design-Build-Test-Learn (DBTL) approach to simultaneously learn how a function is encoded in biology and attempt to engineer it. Many software and hardware platforms have been developed to automate, optimize and algorithmically perform each step of the DBTL cycle. However, there are many fewer options for automating the build step. Build typically involves deoxyribonucleic acid (DNA) assembly, which remains manual, low throughput and unreliable in most cases and limits our ability to advance the science and engineering of biology. Here, we present AssemblyTron, an open-source Python package to integrate j5 DNA assembly design software outputs with build implementation in Opentrons liquid handling ro

SUBMITTER: Bryant JA 

PROVIDER: S-EPMC9832943 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature