IQGAP1 and RNA Splicing in the Context of Head and Neck via Phosphoproteomics.

ABSTRACT: IQGAP1 (IQ motif-containing GTPase-activating protein 1) scaffolds several signaling pathways in mammalian cells that are implicated in carcinogenesis, including the RAS and PI3K pathways that involve multiple protein kinases. IQGAP1 has been shown to promote head and neck squamous cell carcinoma (HNSCC); however, the underlying mechanism(s) remains unclear. Here, we report a mass spectrometry-based analysis identifying differences in phosphorylation of cellular proteins in vivo and in vitro in the presence or absence of IQGAP1. By comparing the esophageal phosphoproteome profiles between Iqgap1^+/+ and Iqgap1^-/- mice, we identified RNA splicing as one of the most altered cellular processes. Serine/arginine-rich splicing factor 6 (SRSF6) was

SUBMITTER: Muehlbauer LK 

PROVIDER: S-EPMC9833422 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature