DNA methylation profile in the whole blood of acute coronary syndrome patients with aspirin resistance.

ABSTRACT:

Background

Aspirin resistance (AR) results in major adverse cardiovascular events, and DNA methylation might participate in the regulation of this pathological process.

Methods

In present study, a sum of 35 patients with AR and 35 non-AR (NAR) controls were enrolled. Samples from 5 AR and 5 NAR were evaluated in an 850 BeadChip DNA methylation assay, and another 30 AR versus 30 NAR were evaluated to validate the differentially methylated CpG loci (DML). Then, qRT-PCR was used to investigate the target mRNA expression of genes at CpG loci. Finally, Gene Ontology (GO) as well as Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to reveal the enriched pathways.

Results

The AR and NAR groups displayed significant differences in DNA methylation at 7707

SUBMITTER: Yu Q 

PROVIDER: S-EPMC9833987 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature