Integrated intracellular organization and its variations in human iPS cells.

ABSTRACT: Understanding how a subset of expressed genes dictates cellular phenotype is a considerable challenge owing to the large numbers of molecules involved, their combinatorics and the plethora of cellular behaviours that they determine^1,2. Here we reduced this complexity by focusing on cellular organization-a key readout and driver of cell behaviour^3,4-at the level of major cellular structures that represent distinct organelles and functional machines, and generated the WTC-11 hiPSC Single-Cell Image Dataset v1, which contains more than 200,000 live cells in 3D, spanning 25 key cellular structures. The scale and quality of this dataset permitted the creation of a generalizable analysis framework to convert raw image data of cells and their structures into dimensionally re

SUBMITTER: Viana MP 

PROVIDER: S-EPMC9834050 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature