NeuroLINCS Proteomics: Defining human-derived iPSC proteomes and protein signatures of pluripotency.

ABSTRACT: The National Institute of Health (NIH) Library of integrated network-based cellular signatures (LINCS) program is premised on the generation of a publicly available data resource of cell-based biochemical responses or "signatures" to genetic or environmental perturbations. NeuroLINCS uses human inducible pluripotent stem cells (hiPSCs), derived from patients and healthy controls, and differentiated into motor neuron cell cultures. This multi-laboratory effort strives to establish i) robust multi-omic workflows for hiPSC and differentiated neuronal cultures, ii) public annotated data sets and iii) relevant and targetable biological pathways of spinal muscular atrophy (SMA) and amyotrophic lateral sclerosis (ALS). Here, we focus on the proteomics and the quality of the developed workflow of

SUBMITTER: Matlock AD 

PROVIDER: S-EPMC9834231 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature