Comparison of metabolites in rumen fluid, urine, and feces of dairy cow from subacute ruminal acidosis model measured by proton nuclear magnetic resonance spectroscopy.

ABSTRACT:

Objective

In this study, metabolites that changed in the rumen fluid, urine and feces of dairy cows fed different feed ratios were investigated.

Methods

Eight Holstein cows were used in this study. Rumen fluid, urine, and feces were collected from the normal concentrate diet (NCD) (Italian ryegrass 80%: concentrate 20% in the total feed) and high concentrate diet (HCD) groups (20%: 80%) of dairy cows. Metabolite analysis was performed using proton nuclear magnetic resonance (NMR) identification, and statistical analysis was performed using Chenomx NMR software 8.4 and Metaboanalyst 4.0.

Results

The two groups of rumen fluid and urine samples were separated, and samples from the same group were aggregated together. On the other hand, the feces samples were not separate

SUBMITTER: Kim HS 

PROVIDER: S-EPMC9834661 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature