Molecular profiles of single circulating tumor cells from early breast cancer patients with different lymph node statuses.

ABSTRACT:

Background

Characterization of early breast cancer circulating tumor cells (CTCs) may provide valuable information on tumor metastasis.

Methods

We used immunomagnetic nanospheres to capture CTCs from the peripheral blood of eight early breast cancer patients and then performed single-cell RNA sequencing using our proposed bead-dd-seq method.

Results

CTCs displayed obvious tumor cell characteristics, such as the activation of oxidative stress, proliferation, and promotion of metastasis. CTCs were clustered into two subtypes significantly correlated with the lymph node metastasis status of patients. CTCs in subtype 1 showed a strong metastatic ability because these CTCs have the phenotype of partial epithelial-mesenchymal transition and enriched transcripts, indicating

SUBMITTER: Pang S 

PROVIDER: S-EPMC9834698 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature