Effect of p38 inhibitor on the proliferation of chicken muscle stem cells and differentiation into muscle and fat.

ABSTRACT:

Objective

Inhibiting the p38 mitogen-activated protein kinase (MAPK) signaling pathway delays differentiation and increases proliferation of muscle stem cells in most species. Here, we aimed to investigate the effect of p38 inhibitor (p38i) treatment on the proliferation and differentiation of chicken muscle stem cells.

Methods

Chicken muscle stem cells were collected from the muscle tissues of Hy-line Brown chicken embryos at embryonic day 18, then isolated by the preplating method. Cells were cultured for 4 days in growth medium supplemented with dimethyl sulfoxide or 1, 10, 20 μM of p38i, then subcultured for up to 4 passages. Differentiation was induced for 3 days with differentiation medium. Each treatment was replicated 3 times.

Results

The proliferation and mRN

SUBMITTER: Ryu M 

PROVIDER: S-EPMC9834727 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature