Dietary fat induced chylomicron-mediated LPS translocation in a bicameral Caco-2cell model.

ABSTRACT:

Background

There is increasing evidence that dietary fat, especially saturated fat, promotes the translocation of lipopolysaccharide (LPS) via chylomicron production in the gut. Chylomicrons can subsequently transport LPS to other parts of the body, where they can induce low-grade chronic inflammation that is linked to various metabolic and gut-related diseases. To identify promising (food) compounds that can prevent or ameliorate LPS-related low-grade inflammation, we developed and optimized a bicameral in vitro model for dietary fat-induced LPS translocation that closely mimics the in vivo situation and facilitates high-throughput screening.

Methods

Caco-2 cells were cultured in monolayers and differentiated to a small intestinal phenotype in 21 days. Thereafter, optimal c

SUBMITTER: Tomassen MMM 

PROVIDER: S-EPMC9835336 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature