Ligation-based assay for variant typing without sequencing: Application to SARS-CoV-2 variants of concern.

ABSTRACT:

Background

COVID-19 prevalence has remained high throughout the pandemic with intermittent surges, due largely to the emergence of genetic variants, demonstrating the need for more accessible sequencing technologies for strain typing.

Methods

A ligation-based typing assay was developed to detect known variants of severe acute respiratory syndrome virus 2 (SARS-CoV-2) by identifying the presence of characteristic single-nucleotide polymorphisms (SNPs). General principles for extending the strategy to new variants and alternate diseases with SNPs of interest are described. Of note, this strategy leverages commercially available reagents for assay preparation, as well as standard real-time polymerase chain reaction (PCR) instrumentation for assay performance.

Results

The

SUBMITTER: Nelson DJ 

PROVIDER: S-EPMC9835417 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature