Microglia and meningeal macrophages depletion delays the onset of experimental autoimmune encephalomyelitis.

ABSTRACT: In multiple sclerosis and the experimental autoimmune encephalomyelitis (EAE) model, both resident microglia and infiltrating macrophages contribute to demyelination as well as spontaneous remyelination. Nevertheless, the specific roles of microglia versus macrophages are unknown. We investigated the influence of microglia in EAE using the colony stimulating factor 1 receptor (CSF-1R) inhibitor, PLX5622, to deplete microglial population and Ccr2^RFP/+ fms^EGFP/+ mice, to distinguish blood-derived macrophages from microglia. PLX5622 treatment depleted microglia and meningeal macrophages, and provoked a massive infiltration of CCR2⁺ macrophages into demyelinating lesions and spinal cord parenchyma, albeit it did not alter EAE chronic phase. In contrast, microgl

SUBMITTER: Montilla A 

PROVIDER: S-EPMC9835747 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature