Simultaneous Sizing and Refractive Index Analysis of Heterogeneous Nanoparticle Suspensions.

ABSTRACT: Rapid and reliable characterization of heterogeneous nanoparticle suspensions is a key technology across the nanosciences. Although approaches exist for homogeneous samples, they are often unsuitable for polydisperse suspensions, as particles of different sizes and compositions can lead to indistinguishable signals at the detector. Here, we introduce holographic nanoparticle tracking analysis, holoNTA, as a straightforward methodology that decouples size and material refractive index contributions. HoloNTA is applicable to any heterogeneous nanoparticle sample and has the sensitivity to measure the intrinsic heterogeneity of the sample. Specifically, we combined high dynamic range k-space imaging with holographic 3D single-particle tracking. This strategy enables long-term tracking by exte

SUBMITTER: Ortiz-Orruno U 

PROVIDER: S-EPMC9835976 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature