Phototank setup and focus stack imaging method for reptile and amphibian specimens (Amphibia, Reptilia).

ABSTRACT: Fluid-preserved reptile and amphibian specimens are challenging to photograph with traditional methods due to their complex three-dimensional forms and reflective surfaces when removed from solution. An effective approach to counteract these issues involves combining focus stack photography with the use of a photo immersion tank. Imaging specimens beneath a layer of preservative fluid eliminates glare and risk of specimen desiccation, while focus stacking produces sharp detail through merging multiple photographs taken at successive focal steps to create a composite image with an extended depth of field. This paper describes the wet imaging components and focus stack photography workflow developed while conducting a large-scale digitization project for targeted reptile and amphibian specim

SUBMITTER: Braker EM 

PROVIDER: S-EPMC9836466 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature