Overexpression of NDST1 Attenuates Fibrotic Response in Murine Adipose-Derived Stem Cells.

ABSTRACT: Adipose-derived stem cells (ADSCs) hold tremendous potential for treating diseases and repairing damaged tissues. Heparan sulfate (HS) plays various roles in cellular signaling mechanisms. The importance of HS in stem cell function has been reported and well documented. However, there has been little progress in using HS for therapeutic purposes. We focused on one of the sulfotransferases, NDST1, which influences overall HS chain extent and sulfation pattern, with the expectation to enhance stem cell function by increasing the N-sulfation level. We herein performed transfections of a green fluorescent protein-vector control and NDST1-vector into mouse ADSCs to evaluate stem cell functions. Overexpression of NDST1 suppressed the osteogenic differentiation of ADSCs. There was no pronounced e

SUBMITTER: Otsuka T 

PROVIDER: S-EPMC9836701 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature