Effects of nitrate and ammonium on assimilation of nitric oxide by Heterosigma akashiwo.

ABSTRACT: The harmful alga Heterosigma akashiwo possesses a hybrid nitrate reductase (NR) enzyme, NR2-2/2HbN, which has the potential to convert NO to nitrate for assimilation into biomass. In previous research, NR transcription in H. akashiwo was induced by nitrate while NR activity was inhibited by ammonium. Here, the capacity of H. akashiwo to use NO in the presence of nitrate and/or ammonium was investigated to understand the regulation of NO assimilation. Continuous cultures of H. akashiwo were acclimated to growth on nitrate, ammonium, or a mixture of both. Aliquots from these cultures were spiked with ¹⁵N-labeled NO. The expression of genes involved in nitrogen assimilation was evaluated, as well as nitrate reductase activity and assimilation of ¹⁵N-labeled nitrogen into

SUBMITTER: Healey EM 

PROVIDER: S-EPMC9837059 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature