Optimization of extracellular matrix for primary human hepatocyte cultures using mixed collagen-Matrigel matrices.

ABSTRACT: Loss of differentiation of primary human hepatocytes (PHHs) ex vivo is a known problem of in vitro liver models. Culture optimizations using collagen type I and Matrigel reduce the dedifferentiation process but are not able to prevent it. While neither of these extracellular matrices (ECMs) on their own correspond to the authentic hepatic ECM, a combination of them could more closely resemble the in vivo situation. Our study aimed to systematically analyze the influence of mixed matrices composed of collagen type I and Matrigel on the maintenance and reestablishment of hepatic functions. Therefore, PHHs were cultured on mixed collagen-Matrigel matrices in monolayer and sandwich cultures and viability, metabolic capacity, differentiation markers, cellular arrangement an

SUBMITTER: Seidemann L 

PROVIDER: S-EPMC9837384 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature