Alkaloid production and response to natural adverse conditions in Peganum harmala: in silico transcriptome analyses.

ABSTRACT: Peganum harmala is a valuable wild plant that grows and survives under adverse conditions and produces pharmaceutical alkaloid metabolites. Using different assemblers to develop a transcriptome improves the quality of assembled transcriptome. In this study, a concrete and accurate method for detecting stress-responsive transcripts by comparing stress-related gene ontology (GO) terms and public domains was designed. An integrated transcriptome for P. harmala including 42 656 coding sequences was created by merging de novo assembled transcriptomes. Around 35 000 transcripts were annotated with more than 90% resemblance to three closely related species of Citrus, which confirmed the robustness of the assembled transcriptome; 4853 stress-responsive transcripts were

SUBMITTER: Jazayeri SM 

PROVIDER: S-EPMC9837557 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature