Interactions between the gut micro-community and transcriptome of Culex pipiens pallens under low-temperature stress.

ABSTRACT:

Background

Culex pipiens pallens (Diptera: Culicidae) can survive at low temperature for long periods. Understanding the effects of low-temperature stress on the gut microflora and gene expression levels in Cx. pipiens pallens, as well as their correlation, will contribute to the study of the overwintering mechanism of Cx. pipiens pallens.

Methods

The gut bacteria were removed by antibiotic treatment, and the survival of Cx. pipiens pallens under low-temperature stress was observed and compared with the control group. Then, full-length 16S rRNA sequencing and the Illumina HiSeq X Ten sequencing platform were used to evaluate the gut microflora and gene expression levels in Cx. pipiens pallens under low-temperature stress.

Results

Under the low-temperature stress of 7 

SUBMITTER: Lv WX 

PROVIDER: S-EPMC9837946 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature