Colonic TRPV4 overexpression is related to constipation severity.

ABSTRACT:

Background

Chronic constipation is prevalent and involves both colon sensitivity and various changes in intestinal bacteria, particularly mucosa-associated microflora. Here we examined regulatory mechanisms of TRPV4 expression by co-culturing colon epithelial cell lines with intestinal bacteria and their derivatives. We also investigated TRPV4 expression in colon epithelium from patients with constipation.

Methods

Colon epithelial cell lines were co-cultured with various enterobacteria (bacterial components and supernatant), folate, LPS, or short chain fatty acids. TRPV4 expression levels and promoter DNA methylation were assessed using pyrosequencing, and microarray network analysis. For human samples, correlation coefficients were calculated and multiple regression analyse

SUBMITTER: Mihara H 

PROVIDER: S-EPMC9838009 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature