Brain endothelium-derived extracellular vesicles containing amyloid-beta induce mitochondrial alterations in neural progenitor cells.

ABSTRACT:

Aim

Elevated brain deposits of amyloid beta (Aβ₄₀) contribute to neuropathology and cognitive dysfunction in Alzheimer's disease (AD). However, the role of the blood-brain barrier (BBB) as an interface for the transfer of Aβ₄₀ from the periphery into the brain is not well characterized. In addition, a substantial population of neural progenitor cells (NPCs) resides in close proximity to brain capillaries that form the BBB. The aim of this study is to understand the impact of brain endothelium-derived extracellular vesicles (EV) containing Aβ₄₀ on metabolic functions and differentiation of NPCs.

Methods

Endothelial EVs were derived from an in vitro model of the brain endothelium treated with 100 nM Aβ₄₀ or PBS. We then analyz

SUBMITTER: Osborne OM 

PROVIDER: S-EPMC9838065 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature